Analysis Note

ControlHela cell lysate

Application

Anti-Yes-associated protein (YAP1) Antibody, clone 4D11.1 detects level of Yes-associated protein (YAP1) & has been published & validated for use in WB & IC.

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Yes-associated protein (YAP1) in HeLa and A431 cells.

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The YAP1 gene encodes a protein that binds to the SH3 (Src homology 3) domain of the Yes proto-oncogene product. As a transcriptional regulator, it can act as a coactivator and a corepressor, and serves as a critical downstream regulatory target in the Hippo signaling pathway. Additionally, it plays a critical role in regulating organ size and in tumor suppression by restricting proliferation and promoting apoptosis. The human YAP1 gene is well conserved among higher eukaryotes, but may not be conseved in yeast. Yes-associated protein expression is almost ubiquitous in adult human tissues, but is not detectable in peripheral blood leukocytes.

Immunogen

GST-tagged recombinant protein corresponding to human Yes-associated protein (YAP1).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ supernatant containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Yes-associated protein (YAP1) in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~65 kDa observed